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Updated: Aug 12, 2026
A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
[Screening and characterization of human phage antibody to hepatitis C core antigen]
1Gene Therapy Research Center, Institute of Infectious Diseases, The 302nd Hospital of PLA, Beijing, 100039, China.
Objective:
To screen and characterize human phage antibody (ScFv) against hepatitis C core antigen.
Methods:
The recombinant phages were panned by core antigen that was coated in a microtiter plate. After three rounds of biopanning, 48 clones were determined specific to core antigen. The specificity of each ScFv colone was determined by ELISA. The coding gene for HCV protein ScFv has been sequenced.
Results:
Phage antibody for HCV core protein had a specific combination character with core antigen of hepatitis C virus. The DNA sequence data showed that the ScFv gene included 774 bp.
Conclusions:
Human single chain antibody to hepatitis C core antigen has been identified by means of the phage display technology.
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