Related Experiment Video
Updated: Jul 24, 2026
Enumeration of Neural Stem Cells Using Clonal Assays
Published on: October 4, 2016
Surface markers expressed by multipotent human and mouse neural progenitor cells include tetraspanins and non-protein
H Klassen1, M R Schwartz, A H Bailey
1Stem Cell Research, Children's Hospital of Orange County, 455 South Main Street, Orange, CA 92868, USA. hklassen@choc.org
Abstract:
Surface molecules play important roles in a wide range of cellular functions, yet little has been reported regarding the expression of such markers by neural stem cells. Here, multipotent human neural progenitor cells (hNPCs) were expanded as a monolayer in the presence of fibroblast/epidermal growth factor, harvested, labeled with monoclonal antibodies, and analyzed by flow cytometry. Positive markers included CD9, CD15, CD81, CD95 (Fas), GD(2) ganglioside, and major histocompatibility complex class I and beta2 microglobulin, as well as low levels of the hematopoietic stem cell marker CD34. Of these, mouse NPCs were positive for CD9, CD15, CD81, and GD(2) ganglioside. The markers reported here have been implicated in a wide range of cellular functions including proliferation, migration, differentiation, apoptosis, and immune recognition.
