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Updated: Aug 2, 2026

Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa
Published on: July 12, 2018
A monoclonal antibody specific for Pseudomonas aeruginosa amidase
C Novo1, A Karmali, A Clemente
1INETI, Departmento de Biotecnologia, UTPAM, Estrada do Paço do Lumiar, 1649-038, Lisboa, Portugal. carlos.novo@mail.ineti.pt
Abstract:
Amidase from Pseudomonas aeruginosa was purified by anionic exchange chromatography and used to immunise female Balb/c mice. Monoclonal antibodies (MAbs) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. A selected IgM subclass MAb was purified from in vitro hybridoma cell line supernatant by a two-step anionic exchange chromatography. The MAb was specific for amidase from P. aeruginosa as determined by Western blotting and recognized the native and denatured forms of the enzyme.
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