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Related Experiment Videos

Progesterone receptor isoforms expression pattern in human astrocytomas.

G González-Agüero1, R Ondarza, A Gamboa-Domínguez

  • 1Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, Coyoacán, México.

Brain Research Bulletin
|October 18, 2001
PubMed
Summary

Progesterone receptors (PR) are present in astrocytomas, with PR-B being the dominant form. Estrogen receptor-alpha is absent in grade IV astrocytomas, suggesting specific hormonal influences in these brain tumors.

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Area of Science:

  • Neuro-oncology
  • Molecular Biology
  • Endocrinology

Background:

  • Progesterone receptors (PR) presence in human astrocytomas is known.
  • The specific expression patterns of PR isoforms in astrocytomas remain uncharacterized.
  • Understanding PR isoform expression is crucial for potential therapeutic strategies.

Purpose of the Study:

  • To investigate the expression patterns of progesterone receptor (PR) isoforms in human astrocytomas (grades III and IV).
  • To determine the predominant PR isoform and its correlation with tumor grade.
  • To assess the expression of estrogen receptor-alpha in astrocytomas.

Main Methods:

  • Analysis of 13 astrocytoma biopsies (6 grade III, 7 grade IV) from adult Mexican patients.
  • Utilized reverse transcription-polymerase chain reaction (RT-PCR) for mRNA analysis.

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  • Employed immunohistochemistry for protein-level expression assessment.

    • Main Results:

    • PR expression was detected at mRNA and protein levels in 66% and 83% of grade III astrocytomas, respectively.
    • 100% of grade IV astrocytomas expressed PR at both mRNA and protein levels.
    • PR-B was the predominant isoform, with higher PR-B than PR-A immunoreactivity in 73% of cases. Estrogen receptor-alpha was found in 33% of grade III but not in grade IV astrocytomas.

    Conclusions:

    • PR-B is the predominant progesterone receptor isoform in human astrocytomas grades III and IV.
    • Estrogen receptor-alpha is notably absent in grade IV astrocytomas.
    • These findings contribute to understanding the hormonal microenvironment in astrocytoma progression.