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Updated: Aug 14, 2026
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
Published on: January 2, 2018
The notch intracellular domain can function as a coactivator for LEF-1
1Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, 19104-6145, USA.
Molecular and Cellular Biology
|October 18, 2001
Summary
Notch signaling
Area of Science:
- Cellular biology
- Molecular biology
- Biochemistry
Background:
- Notch signaling is initiated by ligand-mediated proteolysis, releasing the Notch intracellular domain (NICD).
- NICD translocates to the nucleus, interacting with CSL to activate transcription.
Purpose of the Study:
- To investigate the interaction between NICD and the transcription factor LEF-1.
- To determine if NICD influences LEF-1 activity and the underlying mechanisms.
Main Methods:
- Analysis of NICD expression and its effect on LEF-1 activity.
- In vitro binding assays to assess protein-protein interactions.
- Promoter activity assays to identify target genes.
Main Results:
- NICD potentiates LEF-1 activity in a context-dependent manner.
- NICD-mediated LEF-1 activation occurs on a distinct set of promoters compared to beta-catenin.
- A weak in vitro association was observed between NICD and LEF-1, suggesting in vivo interaction.
Conclusions:
- NICD acts as a novel nuclear target of Notch signaling.
- NICD functions as a new coactivator for the transcription factor LEF-1.
- This interaction is independent of canonical Wnt or Notch pathway components.
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