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Updated: Aug 8, 2026

Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions
Published on: November 12, 2017
[The effect of low intensity electromagnetic irradiation on hydration of DNA film]
Iu A Kim1, M M Montrel', V R Akoev
1Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, 142290 Russia. yuakim@mail.ru
Abstract:
By using the IR-spectroscopy it has been shown that electromagnetic radiation (frequency 8.15-10.0 GHz, energy flux density 5 microWt/cm2) reduces the rate of water desorption from DNA films. It was found that the irradiation of samples with high humidity did not change spectral characteristics of DNA molecules in the range of 900-4000 cm-1, that means their molecular structure remains intact. At the same time the irradiation changed conformation liability of these biopolymeric molecules, that is their ability of conformational transformations under the influence of outer factors. Drying of non-irradiated humid films induced rapid (for a few minutes) transition of DNA from B to A conformational state, whereas in the irradiated films this transition took several hours after humidity reducing.
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