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Multidimensional on-line solid-phase extraction (SPE) using restricted access materials (RAM) in combination with

K S Boos1, C T Fleischer

  • 1Institute of Clinical Chemistry, University Hospital Grosshadern, Munich, Germany. boos@klch.med.uni-muenchen.de

Fresenius' Journal of Analytical Chemistry
|October 19, 2001
PubMed
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A novel Six-S ProcEdure (Six-SPE) uses restricted access material (RAM) and molecular imprinted polymers (MIP) for complex fluid sample processing. This method efficiently removes interfering substances and selectively isolates target analytes for analysis.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Biomolecular Analysis

Background:

  • Complex biological fluids present significant challenges for direct analytical measurement due to interfering matrix components.
  • Traditional sample preparation methods often involve multiple steps, leading to analyte loss and increased processing time.
  • Selective recognition and isolation of target analytes from intricate matrices remain a critical bottleneck in many analytical workflows.

Purpose of the Study:

  • To introduce a novel, multidimensional Solid Phase Extraction (SPE) platform for the efficient processing of complex fluid samples.
  • To develop a streamlined sample preparation technique that combines size-exclusion and molecular imprinting for enhanced analyte isolation.
  • To demonstrate the platform's capability in preparing samples for on-line analysis of specific target analytes.

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Main Methods:

  • The Six-S ProcEdure (Six-SPE) utilizes a sequential combination of restricted access material (RAM) columns and molecular imprinted polymer (MIP) columns.
  • A size-selective separation on the RAM column removes macromolecular interferents while retaining low molecular weight compounds.
  • A subsequent solvent-switch transfers the retained analytes to the MIP column for selective molecular recognition and binding.

Main Results:

  • The Six-SPE platform effectively removes interfering matrix components from complex aqueous samples like plasma, urine, and milk.
  • The method creates optimal conditions for selective analyte binding by tailor-made MIPs.
  • Coupling Six-SPE with HPLC enabled on-line analysis of the analgesic drug Tramadol in human plasma.

Conclusions:

  • The Six-S ProcEdure (Six-SPE) offers a powerful and versatile multidimensional SPE approach for complex sample matrices.
  • This technique significantly simplifies sample preparation, enhancing selectivity and efficiency for on-line analytical measurements.
  • The demonstrated application in Tramadol analysis highlights the platform's potential for therapeutic drug monitoring and other clinical applications.