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Related Experiment Videos

Pulsed electrospray for mass spectrometry.

Y Lu1, T F Zhou, T W Shui

  • 1Department of Chemistry, Fudan University, Shanghai, China.

Analytical Chemistry
|October 19, 2001
PubMed
Summary

A novel pulsed electrospray ionization (ESI) technique was developed, enabling controlled on-and-off spray processes. This innovation allows stable electrospray at lower flow rates, potentially reducing sample consumption in mass spectrometry.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Conventional electrospray ionization (ESI) is a widely used technique for generating ions.
  • Controlling the ESI process, especially at low flow rates, can be challenging.

Purpose of the Study:

  • To develop and demonstrate a novel pulsed electrospray ionization (ESI) technique.
  • To investigate the feasibility of achieving controlled on-and-off spray processes.
  • To explore the potential for reduced sample consumption.

Main Methods:

  • Development of a homemade pulsed high-voltage (HV) circuit integrated with conventional ESI.
  • Monitoring of the pulsed spray using a digital microscope and an oscilloscope.
  • Characterization of pulsed ion current and pulsed mass spectra.

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Main Results:

  • Successful operation of the pulsed ESI device with a clear on-and-off spray process.
  • Demonstration of pulsed ion current and pulsed mass spectra.
  • Achievement of stable electrospray at lower flow rates compared to conventional ESI under identical conditions.

Conclusions:

  • The developed pulsed ESI technique offers precise control over the ionization process.
  • This method enables stable electrospray at reduced sample flow rates, indicating potential for decreased sample usage.
  • The pulsed ESI device is a promising advancement for mass spectrometry applications requiring minimal sample volumes.