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Updated: Aug 15, 2026

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
Published on: March 5, 2017
Lipid membrane reorganization induced by chemical recognition
J A Last1, T A Waggoner, D Y Sasaki
1Biomolecular Materials and Interfaces Department, Sandia National Laboratories, Albuquerque, New Mexico 87185, USA.
Abstract:
Nanoscale structural reorganization of a lipid bilayer membrane induced by a chemical recognition event has been imaged using in situ atomic force microscopy (AFM). Supported lipid bilayers, composed of distearylphosphatidylcholine (DSPC) and a synthetic lipid functionalized with a Cu(2+) receptor, phase-separate into nanoscale domains that are distinguishable by the 9 A height difference between the two molecules. Upon binding of Cu(2+) the electrostatic nature of the receptor changes, causing a dispersion of the receptor molecules and subsequent shrinking of the structural features defined by the receptors in the membrane. Complete reversibility of the process was demonstrated through the removal of metal ions with EDTA.
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