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Related Experiment Videos

A Ras subfamily GTPase shows cell cycle-dependent nuclear localization.

B W Sutherland1, G B Spiegelman, G Weeks

  • 1Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada.

EMBO Reports
|October 19, 2001
PubMed
Summary
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Dictyostelium RasB protein, unlike other Ras proteins, is found in the nucleus. RasB

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Ras subfamily proteins typically function in signal transduction at the plasma membrane.
  • Their role in intracellular processes is less understood.

Purpose of the Study:

  • To investigate the subcellular localization and function of the Dictyostelium Ras subfamily protein RasB.
  • To explore the novel roles of RasB beyond plasma membrane signaling.

Main Methods:

  • Immunofluorescence microscopy to determine RasB localization during the cell cycle.
  • Analysis of rasB- knockout cells and cells overexpressing activated RasB.

Main Results:

  • RasB predominantly localizes to the nucleus during interphase.

Related Experiment Videos

  • RasB exhibits diffuse cellular localization during mitosis and cytokinesis.
  • rasB- cells show severe growth defects.
  • Overexpression of activated RasB leads to multinucleation.

    • Conclusions:

    • RasB possesses a novel nuclear localization and function.
    • RasB plays a critical role in nuclear division and cell cycle progression.
    • RasB's function extends beyond the canonical plasma membrane-associated Ras signaling pathways.