Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
  1. Home
  3. Research Domains
  1. Home
  3. Research Domains

Related Concept Videos

  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • Multicellular Gastric Cancer Spheroids Recapitulate Growth Pattern And Differentiation Phenotype Of Human Gastric Carcinomas.
  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • Multicellular Gastric Cancer Spheroids Recapitulate Growth Pattern And Differentiation Phenotype Of Human Gastric Carcinomas.

    • Related Experiment Videos

    Multicellular gastric cancer spheroids recapitulate growth pattern and differentiation phenotype of human gastric carcinomas.

    B Mayer1, G Klement, M Kaneko

    • 1Molecular and Cellular Biology Research, University of Toronto, Sunnybrook and Women's College Health Sciences Centre, Toronto, Ontario, Canada.

    Gastroenterology
    |October 19, 2001

    View abstract on PubMed

    Summary
    This summary is machine-generated.

    A new multicellular gastric cancer spheroid model mimics patient tumors, offering a better way to study gastric cancer and test new therapies for this difficult disease.

    Related Experiment Videos

    Area of Science:

    • Oncology
    • Cancer Biology
    • Preclinical Models

    Background:

    • Advanced gastric cancer presents a significant clinical challenge with poor prognosis.
    • Current chemotherapeutic drugs show limited efficacy against advanced gastric cancer.
    • Improved preclinical models are crucial for developing more effective gastric cancer therapies.

    Purpose of the Study:

    • To establish and validate an in vitro multicellular gastric cancer spheroid model.
    • To compare the characteristics of the spheroid model with corresponding in vivo xenografts.
    • To assess the utility of the spheroid model for recapitulating tumor architecture and biology.

    Main Methods:

    • Established an in vitro multicellular gastric cancer spheroid model using the liquid overlay technique.
    • Compared spheroid characteristics with parental gastric carcinomas and their xenografts in immunodeficient mice.
    • Analyzed morphologic differentiation, mucin, and E-cadherin expression in spheroids.

    Main Results:

    • 71% of gastric cancer cell lines formed spheroids reflecting parental tumor growth characteristics.
    • Spheroids from differentiated tumors showed recapitulated architecture, mucin, and E-cadherin expression.
    • Spheroids from poorly differentiated tumors formed compact structures and showed distinct molecular profiles.

    Conclusions:

    • Multicellular gastric cancer spheroids can recapitulate some complexity of in vivo tumors.
    • These spheroids represent a physiologically relevant model for studying gastric cancer biology.
    • The model holds potential for testing novel therapeutic strategies against gastric cancer.