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Anchored reference loci in loblolly pine (Pinus taeda L.) for integrating pine genomics
G R Brown1, E E Kadel, D L Bassoni
1Institute of Forest Genetics, Pacific Southwest Research Station, U.S. Department of Agriculture Forest Service, Davis, California 95616, USA.
Genetics
|October 19, 2001
Summary
Researchers identified 35 anchor loci from loblolly pine (Pinus taeda L.) expressed sequence tag polymorphisms (ESTPs). These ESTPs serve as valuable landmarks for comparative genetic mapping across pine species, aiding genome organization studies.
Area of Science:
- Plant Genetics
- Genomics
- Forestry
Background:
- Comparative mapping relies on anchored reference loci to identify conserved chromosomal segments.
- Synthesizing genetic maps from multiple sources requires reliable landmarks.
Purpose of the Study:
- To evaluate expressed sequence tag polymorphisms (ESTPs) from loblolly pine (Pinus taeda L.) as potential anchored reference loci.
- To facilitate comparative mapping and genome evolution studies in pines.
Main Methods:
- Assayed 90 ESTPs for amplification and polymorphism across six pedigrees, including different subgenera of Pinus and Douglas-fir.
- Performed comparative mapping between loblolly pine and slash pine (P. elliottii Englm.).
Main Results:
- On average, 89% of ESTP primer sets amplified in Pinus species, with lower amplification in Douglas-fir (22%).
- Polymorphisms were detected in 37-61% of ESTPs within pedigrees.
- Comparative mapping confirmed conserved locations for ESTPs between loblolly and slash pine, with no significant synteny disruptions.
- Thirty-five ESTPs met the criteria for anchor loci.
Conclusions:
- Established ESTPs function effectively as anchor loci for comparative mapping in pines.
- These anchor loci will unify genetic mapping efforts, enabling a "generic" pine map.
- Provides a foundation for understanding pine genome organization and evolutionary relationships.