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CD34(+) cell collection efficiency does not correlate with the pre-leukapheresis hematocrit.
1The Hematopoietic Stem Cell Transplant Program, Division of Hematology/Oncology, Department of Internal Medicine, Northwestern University Medical School and The Robert H Lurie Comprehensive Cancer Center of Northwestern University, Chicago, IL 60611, USA.
Bone Marrow Transplantation
|October 19, 2001
Summary
Pre-apheresis hematocrit (Hct) does not impact CD34(+) stem cell collection efficiency during leukapheresis. Clinical factors, not Hct levels, should determine minimum acceptable values for this procedure.
Area of Science:
- Hematology
- Transfusion Medicine
- Cellular Therapy
Background:
- Hematocrit (Hct) is a common parameter monitored before apheresis procedures.
- The influence of pre-apheresis Hct on CD34(+) cell collection efficiency (CE) is not well-established.
Purpose of the Study:
- To investigate the relationship between pre-apheresis hematocrit and CD34(+) cell collection efficiency using the Fenwal CS 3000 Plus cell separator.
Main Methods:
- Retrospective review of 177 large-volume leukapheresis procedures from 91 patients.
- Analysis of pre-apheresis hematocrit, peripheral blood CD34(+) cell counts, and collection efficiency.
Main Results:
- No significant correlation was found between pre-apheresis Hct and CD34(+) cell collection efficiency (r² = 0.0034, P = 0.44).
- No significant correlation was observed between Hct and the total CD34(+) cell quantity collected (r² = 0.0040, P = 0.40).
- Collection efficiencies were comparable across different Hct ranges (<0.25, 0.25-0.299, and ≥0.30).
Conclusions:
- Pre-apheresis hematocrit levels do not appear to affect stem cell collection efficiency in leukapheresis.
- Minimum acceptable Hct or hemoglobin levels for leukapheresis should be based on individual clinical circumstances rather than a universal threshold.