Related Experiment Videos
[Storage of human bone marrow at - 196 degrees C]
Acta Haematologica Polonica
|July 1, 1975
Summary
Dimethyl sulfoxide (DMSO) is a more effective cryoprotective agent than glycerol for storing human bone marrow at -196°C. This finding is based on cell counts, viability, myelograms, and lymphocyte blastic transformation.
Area of Science:
- Cryobiology
- Hematology
- Cell Biology
Background:
- Human bone marrow storage is crucial for transplantation and research.
- Cryoprotective agents are essential for preserving cell viability during low-temperature storage.
- Dimethyl sulfoxide (DMSO) and glycerol are commonly used cryoprotectants.
Purpose of the Study:
- To compare the efficacy of DMSO and glycerol as cryoprotective agents for human bone marrow.
- To evaluate the impact of cryopreservation on nucleated cell count, viability, and cellular function.
Main Methods:
- Human bone marrow samples were cryopreserved at -196°C using DMSO or glycerol.
- Post-thaw analysis included nucleated cell counts, viability assays, myelograms, and lymphocyte blastic transformation assays.
Main Results:
- Dimethyl sulfoxide (DMSO) demonstrated superior cryoprotective properties compared to glycerol.
- Preservation with DMSO resulted in higher nucleated cell counts and viability.
- DMSO maintained better cellular integrity and function, as indicated by myelograms and lymphocyte responses.
Conclusions:
- DMSO is a more effective cryoprotective agent for human bone marrow storage at -196°C than glycerol.
- Optimal cryopreservation methods are vital for maintaining the therapeutic potential of bone marrow grafts.
- These findings support the use of DMSO for long-term human bone marrow banking.