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Modified method for analysis of serum iron.

E Horak, D C Hohnadel, F W Sunderman

    Annals of Clinical and Laboratory Science
    |July 11, 1975
    PubMed
    Summary
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    This study presents a new, accurate method for measuring serum iron. The improved assay avoids interferences common in direct spectrophotometry, ensuring reliable results for clinical diagnostics.

    Area of Science:

    • Clinical Chemistry
    • Analytical Biochemistry

    Background:

    • Accurate serum iron analysis is crucial for diagnosing various medical conditions.
    • Existing direct spectrophotometric methods can be prone to interference, affecting result reliability.

    Purpose of the Study:

    • To develop and validate a simple, rapid, and precise method for routine serum iron analysis in clinical laboratories.
    • To compare the performance of the new method against a direct spectrophotometric approach.

    Main Methods:

    • Serum proteins were precipitated using trichloroacetic acid-hydrochloric acid.
    • Iron(III) was reduced to Iron(II) using sodium ascorbate.
    • Iron(II) reacted with ferrozine, forming a complex measured spectrophotometrically at 562 nm.

    Main Results:

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    • The improved method demonstrated close agreement with the direct method in 93.5% of 183 serum samples analyzed.
    • Discrepancies in the remaining samples were attributed to interferences like hemolysis, lipemia, jaundice, and sample degradation.

    Conclusions:

    • The developed method offers a reliable and convenient alternative for routine serum iron determination.
    • Deproteinization effectively eliminates interferences encountered in direct spectrophotometric assays, enhancing diagnostic accuracy.