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Fluorescence microscopy test in porphyrias, photodermatoses and lead exposed persons
Archiv Fur Dermatologische Forschung
|July 18, 1975
Summary
Fluorescence microscopy detects fluorescing erythrocytes (FE) in various conditions. Elevated FE levels confirm erythropoietic protoporphyria but require caution for carrier detection in genetic studies.
Area of Science:
- Hematology
- Biochemistry
- Medical Diagnostics
Background:
- Fluorescence microscopy is utilized to detect fluorescing erythrocytes (FE).
- FE levels can indicate various hematological and metabolic conditions.
- Accurate interpretation is crucial for clinical diagnosis and genetic studies.
Purpose of the Study:
- To evaluate the diagnostic utility of fluorescence microscopy for detecting FE.
- To establish reference FE levels in different patient groups and controls.
- To assess the test's reliability in diagnosing erythropoietic protoporphyria and identifying carriers.
Main Methods:
- Peripheral blood samples were diluted with saline.
- 200 high power fields were inspected per sample.
- Results were quantified as FE per 100,000 red blood cells.
Main Results:
- Minimal FE detected in controls, porphyria cutanea tarda, and other photodermatoses.
- High mean FE in erythropoietic protoporphyria (10600).
- Elevated FE in lead poisoning (1032), lead exposure (48.2), sideropenic anemia (123), and pellagra (8.1).
Conclusions:
- Fluorescence microscopy is valuable for confirming erythropoietic protoporphyria diagnosis.
- Caution is advised when using this test for latent carrier detection in genetic studies.
- FE levels provide a quantitative measure for differentiating various conditions.