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Related Experiment Videos

NO2 interfacial transfer is reduced by phospholipid monolayers.

L M Connor1, A Bidani, J Goerke

  • 1Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas 77555, USA.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|October 20, 2001
PubMed
Summary

Pulmonary surfactant, particularly dipalmitoyl-sn-3-glycero-phosphocholine, significantly resists nitrogen dioxide (NO2) absorption in the lungs. This resistance depends on the phospholipid film

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Area of Science:

  • Pulmonary Physiology
  • Environmental Health
  • Biophysics

Background:

  • Nitrogen dioxide (NO2) is a common air pollutant with detrimental respiratory effects.
  • NO2 absorption in the lungs involves reactions at the air-epithelial lining fluid interface.
  • The role of pulmonary surfactant in modulating NO2 absorption kinetics is not fully understood.

Purpose of the Study:

  • To investigate the impact of interfacial pulmonary surfactant on the absorption of nitrogen dioxide (NO2).
  • To determine if surfactant composition and compression affect NO2 absorption resistance.

Main Methods:

  • Development of a specialized apparatus to expose compressed phospholipid monolayers to NO2.
  • Varied compression levels of monolayers composed of dipalmitoyl-sn-3-glycero-phosphocholine and unsaturated phospholipids.

Related Experiment Videos

  • Measurement of NO2 absorption kinetics across these interfacial films.

    • Main Results:

    • Compressed monolayers rich in 1,2-dipalmitoyl-sn-3-glycero-phosphocholine significantly impeded NO2 absorption.
    • This resistance was observed even at surface tensions exceeding in vivo levels.
    • Monolayers of pure unsaturated phospholipids did not significantly alter NO2 absorption despite surface tension reduction.

    Conclusions:

    • Phospholipid monolayers demonstrably limit NO2 absorption, with resistance linked to film physicochemical properties.
    • The findings suggest pulmonary surfactant influences the intrapulmonary distribution of inhaled NO2.
    • This highlights a potential mechanism by which lung surfactant affects gas pollutant behavior.