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Cloning of bovine CD34 cDNA

J H Zhou1, H Hikono, M Ohta

  • 1Department of Immunology, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.

The Journal of Veterinary Medical Science
|October 20, 2001
PubMed

Insights

Researchers cloned bovine CD34 cDNA, revealing its nucleotide and amino acid sequences. This bovine CD34 protein shares significant identity with human, canine, and murine CD34, aiding stem cell research.

Area of Science:

  • Molecular Biology
  • Immunology
  • Veterinary Science

Background:

  • CD34 is a cell surface glycoprotein crucial for identifying hematopoietic stem and progenitor cells.
  • Monoclonal antibodies targeting CD34 are vital tools in human, murine, and canine research.
  • Understanding CD34 across species facilitates comparative studies in stem cell biology.

Purpose of the Study:

  • To clone and sequence the complementary DNA (cDNA) encoding bovine CD34.
  • To determine the sequence identity of bovine CD34 compared to homologous proteins in other species.
  • To investigate the expression pattern of CD34 RNA in fetal bovine tissues.

Main Methods:

  • CDNA cloning and nucleotide sequencing of the bovine CD34 gene.
  • Bioinformatic analysis to compare deduced amino acid sequences with human, murine, and canine CD34.
  • Northern blot hybridization to detect CD34 RNA expression in fetal calf tissues.

Main Results:

  • The cDNA encoding bovine CD34 was successfully cloned and sequenced.
  • The deduced bovine CD34 amino acid sequence showed 61.1% identity to human, 56.0% to murine, and 66.1% to canine CD34.
  • CD34 RNA expression was detected in the fetal calf cerebrum, spleen, heart, and lung.

Conclusions:

  • The characterization of bovine CD34 provides a valuable resource for comparative immunology and stem cell research.
  • The identified sequence similarities suggest conserved functions of CD34 across mammalian species.
  • The expression of CD34 in various fetal bovine tissues indicates potential roles beyond hematopoietic lineages.

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