Related Experiment Video

Updated: May 5, 2026

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
10:40

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 11, 2013

22.1K

Histones H2a, H2b, H3, and H4 form a tetrameric complex in solutions of high salt

H Weintraub, K Palter, F Van Lente

    Cell
    |September 1, 1975
    PubMed

    More Related Videos

    Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones09:26

    Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

    Published on: March 26, 2017

    10.5K
    In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays08:16

    In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

    Published on: December 29, 2021

    2.5K

    Related Concept Videos

    The Nucleosome02:33

    The Nucleosome

    DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
    DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
    15.0K
    The Nucleosome Core Particle02:10

    The Nucleosome Core Particle

    Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
    The paradox
    Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
    12.2K
    Histone Modification02:32

    Histone Modification

    The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
    Acetylation
    The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
    14.7K
    Histone Variants at the Centromere02:30

    Histone Variants at the Centromere

    Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
    4.0K
    The Nucleosome01:19

    The Nucleosome

    Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
    In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
    3.9K
    The Nucleosome Core Particle01:12

    The Nucleosome Core Particle

    Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
    Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
    2.6K