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Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila
Published on: June 13, 2018
DNA homologies among Drosophila species and a related genus
Abstract:
Deoxyribonucleic acids of members of the family Drosophilidae; e.g., D. melanogaster, D. robusta, D. pellewae, D, immigrans, D. mcclintockae, D. calloptera, C. procnemis and from a tissue culture cells of D. melanogaster have been compared with respect to base composition, heterogenecity, and nucleotide sequence homology. Considerable heterogeneity exists in DNA's from 3rd instar larvae and tissue culture cells. The DNA base composition of adult species ranges from 33-42 moles percent GC; in addition a polydAT component is apparent in larval DNA's. There are about 21 and 29 percent intragenomic homology in DNA's of D. melanogaster and D. immigrans, respectively. Relatively large differences were revealed in the nucleotide sequences of several species by DNA hybridization and thermal stability studies.
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