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Related Experiment Videos

Alanine-induced amino acid interrelationships.

A A Rossini, T T Aoki, O P Ganda

    Metabolism: Clinical and Experimental
    |October 11, 1975
    PubMed
    Summary

    Administering large amounts of alanine significantly alters amino acid and substrate levels in the body. This study observed changes in hormones and key metabolic indicators following alanine intake.

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    Area of Science:

    • Biochemistry
    • Human Physiology
    • Metabolic Studies

    Background:

    • Alanine is a non-essential amino acid with various metabolic roles.
    • Understanding alanine's impact on other metabolites and hormones is crucial for metabolic research.

    Purpose of the Study:

    • To investigate the metabolic and hormonal effects of administering large quantities of alanine.
    • To analyze changes in plasma and whole blood amino acid profiles.
    • To assess alterations in key substrates and hormones.

    Main Methods:

    • Six healthy subjects participated in the study.
    • Alanine was administered orally and intravenously (10g dose over 60 minutes).
    • Blood samples were collected at 30-minute intervals for 2.5 hours to measure hormones and substrates.

    Main Results:

    • Significant increases in plasma levels of threonine, serine, glutamine, proline, glycine, and alpha-amino-n-butyric acid were observed.
    • Whole blood levels of leucine, valine, and isoleucine decreased.
    • Plasma glucagon increased, while insulin only rose significantly after oral alanine administration.
    • Free fatty acids and glycerol decreased; lactate and pyruvate increased. Glucose levels remained unchanged.

    Conclusions:

    • Large alanine administration induces significant changes in amino acid profiles.
    • Alanine affects various metabolic substrates and hormone levels, including glucagon and insulin.
    • These findings highlight alanine's role in modulating human metabolism.

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