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Measles virus-specified polypeptides in infected cells
Archives of Virology
|January 1, 1979
Summary
This study identifies six major structural polypeptides in measles virus-infected cells, revealing most are synthesized at their final length. A 65,000-dalton nonstructural polypeptide may be a precursor to structural polypeptide 5.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Measles virus (MeV) is a significant human pathogen.
- Understanding MeV protein synthesis is crucial for antiviral strategies.
Purpose of the Study:
- To analyze the synthesis of wild-type measles virus-specified polypeptides.
- To identify structural and nonstructural viral proteins and their precursors.
Main Methods:
- Polyacrylamide slab-gel electrophoresis was used to analyze viral polypeptides.
- Experiments included pulse-chase, synchronized protein synthesis, and proteolytic enzyme inhibitors.
- Vero cells were used as the host system.
Main Results:
- Six major structural polypeptides (L, G, 2, NP, 5, M) were identified.
- Most structural polypeptides were synthesized at their final length.
- A 65,000-dalton nonstructural polypeptide may be a precursor to structural polypeptide 5.
Conclusions:
- The study characterizes the synthesis and potential precursor-product relationships of measles virus polypeptides.
- Polypeptide M exhibits sensitivity to trypsin, with altered molecular weight in TLCK-treated cells.