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Updated: Oct 3, 2026

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
Published on: June 9, 2017
Reconstitution of G protein-coupled receptors with recombinant G protein alpha and beta gamma subunits
William E McIntire1, Chang-Seon Myung, Gavin MacCleery
1Department of Pharmacology, University of Virginia Health System, Charlottesville, Virginia 22908, USA.
Abstract:
The methods outlined in this article describe experiments that can probe the first steps in receptor:G protein interaction using defined, recombinant receptors and G proteins. The protocols have the advantages that the receptors are inserted properly in a cell membrane and that the investigator has complete control of the proteins reconstituted with the receptor. Specific mutations in the receptors or G proteins are studied easily and the protocols allow precise examination of the stoichiometry of the receptor:alpha:beta gamma interaction.
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