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Pregnancy and estradiol decrease GTPase activity in the guinea pig uterine artery.

I A Buhimschi1, G Hall, L P Thompson

  • 1Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201-1559, USA.

American Journal of Physiology. Heart and Circulatory Physiology
|October 23, 2001
PubMed
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Pregnancy and estradiol reduce GTPase activity in uterine arteries, enhancing cGMP release. This G protein signaling change may explain cardiovascular adaptations during pregnancy.

Area of Science:

  • Cardiovascular Physiology
  • Endocrinology
  • Molecular Signaling

Background:

  • Pregnancy significantly alters cardiovascular function, redistributing cardiac output.
  • The underlying molecular mechanisms, particularly estrogen's role in G protein signaling, are not fully understood.

Purpose of the Study:

  • To investigate the impact of pregnancy and estrogen on G protein-mediated signal transduction in guinea pig arteries.
  • To determine if altered signaling in uterine arteries contributes to pregnancy-induced cardiovascular changes.

Main Methods:

  • Assayed high-affinity GTPase activity in aortas, uterine arteries (UAs), and mesenteric arteries (MAs) from pregnant, nonpregnant, and ovariectomized guinea pigs.
  • Measured cyclic guanosine monophosphate (cGMP) generation in response to acetylcholine (ACh) with or without cholera toxin (CTX).

Related Experiment Videos

Main Results:

  • Pregnancy decreased GTPase activity in UAs but not in aortas or MAs.
  • 17beta-estradiol treatment reduced UA GTPase activity.
  • ACh increased cGMP in pregnant UAs; CTX mimicked this effect in nonpregnant UAs, indicating reduced GTPase activity of a CTX-sensitive G protein.

Conclusions:

  • Pregnancy and estradiol decrease GTPase activity of a CTX-sensitive G protein in uterine arteries.
  • This alteration enhances receptor-mediated cGMP release, potentially contributing to cardiovascular adaptations during pregnancy.