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Studies on polyamine biosynthesis in Euglena gracilis.

A Aleksijevic, J Grove, F Schuber

    Biochimica Et Biophysica Acta
    |November 22, 1979
    PubMed
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    Euglena gracilis contains five polyamines, including putrescine, spermidine, and norspermine. Putrescine biosynthesis occurs via L-ornithine decarboxylation, with enzymes similar to those in animal cells.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Algal Metabolism

    Background:

    • Polyamines are essential molecules found in all living organisms.
    • Understanding polyamine biosynthesis in diverse species like Euglena gracilis is crucial for cellular function research.
    • Previous studies have identified various polyamines in different organisms, but their specific roles and pathways in algae require further elucidation.

    Purpose of the Study:

    • To identify and quantify the polyamines present in Euglena gracilis (strain Z).
    • To investigate the biosynthetic pathway of putrescine in Euglena gracilis.
    • To characterize the key enzymes involved in polyamine synthesis and compare them to known enzymes from other organisms.

    Main Methods:

    • High-pressure cation-exchange chromatography for polyamine separation and identification.

    Related Experiment Videos

  • Enzyme activity assays to determine the presence and characteristics of ornithine decarboxylase and S-adenosylmethionine decarboxylase.
  • Biochemical analysis of L-ornithine and arginine decarboxylation pathways.

    • Main Results:

    • Five polyamines were identified: 1,3-diaminopropane, putrescine, norspermidine, spermidine, and norspermine.
    • Putrescine biosynthesis in Euglena gracilis proceeds via L-ornithine decarboxylation.
    • Arginine decarboxylase activity was not detected.
    • The identified ornithine decarboxylase and S-adenosylmethionine decarboxylase enzymes exhibit properties similar to those found in animal and yeast cells.

    Conclusions:

    • Euglena gracilis possesses a distinct polyamine profile with a specific biosynthetic route for putrescine.
    • The enzymatic machinery for polyamine synthesis in this alga shares similarities with eukaryotes from different kingdoms.
    • A proposed biosynthetic scheme integrates the identified polyamines and their synthesis pathway in Euglena gracilis.