Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Determination

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Motivations for Self-Harm in Young People and Their Correlates: A Systematic Review.

Clinical child and family psychology review·2025

Same author

<i>Kgaria</i> (<i>Boletaceae, Boletoideae</i>) <i>gen. nov</i>. in Australia: Neither a <i>Tylopilus</i> nor a <i>Porphyrellus</i>.

Fungal systematics and evolution·2024

Same author

Bridging the age gap in breast cancer: impact of omission of breast cancer surgery in older women with oestrogen receptor-positive early breast cancer on quality-of-life outcomes.

The British journal of surgery·2021

Same author

Bridging the age gap in breast cancer: cluster randomized trial of two decision support interventions for older women with operable breast cancer on quality of life, survival, decision quality, and treatment choices.

The British journal of surgery·2021

Same author

Breast cancer surgery in older women: outcomes of the Bridging Age Gap in Breast Cancer study.

The British journal of surgery·2020

Same author

Biosensing by Learning: Cancer Detection as Iterative optimization.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2018

Search research articles

Related Experiment Video

Updated: Jul 11, 2026

Chicken Embryo Spinal Cord Slice Culture Protocol

Chicken Embryo Spinal Cord Slice Culture Protocol

Published on: March 25, 2013

Expression of slit-2 and slit-3 during chick development.

G Holmes¹, L Niswander

¹Howard Hughes Medical Institute, Memorial Sloan-Kettering Cancer Center, Molecular Biology Program, New York, NY 10021, USA. g-holmes@ski.mskcc.org

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|October 23, 2001

Summary

Chick slit-2 and slit-3 genes guide neuronal development and are expressed in the developing limb. Their distinct expression patterns suggest roles in limb development, including cartilage and muscle formation.

More Related Videos

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Published on: January 17, 2016

Creating Avian Forebrain Chimeras to Assess Facial Development

Creating Avian Forebrain Chimeras to Assess Facial Development

Published on: February 18, 2021

Related Experiment Videos

Last Updated: Jul 11, 2026

Chicken Embryo Spinal Cord Slice Culture Protocol

Chicken Embryo Spinal Cord Slice Culture Protocol

Published on: March 25, 2013

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Published on: January 17, 2016

Creating Avian Forebrain Chimeras to Assess Facial Development

Creating Avian Forebrain Chimeras to Assess Facial Development

Published on: February 18, 2021

Area of Science:

Developmental Biology
Molecular Biology
Genetics

Background:

Slit proteins and Robo receptors guide neuronal axons and neurons.
Slit genes are also found in developing limbs, but their function there is unclear.

Purpose of the Study:

To characterize the expression of chick slit-2 and slit-3 in the developing limb.
To provide a basis for understanding slit functions in limb development.

Main Methods:

Isolation and characterization of chick slit-2 and slit-3 gene expression.
Analysis of expression patterns in chick embryos, focusing on the developing limb and neural tube.

Main Results:

Chick slit-2 and slit-3 show expression patterns similar to mammalian homologues, especially in the neural tube.
In the limb, slit-2 is found in peripheral mesenchyme and muscle precursors, while slit-3 is in the chondrogenic core.
Later, both genes are expressed in interdigital mesenchyme, periosteal cells, and under the epidermis; slit-3 is in chondrocytes, and slit-2 in muscle and near joints.

Conclusions:

Chick slit-2 and slit-3 expression patterns in the limb suggest involvement in various developmental processes.
These findings lay the groundwork for further investigation into the specific roles of slit genes in limb morphogenesis.