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[Dominant selecting culture medium of Peucedanum decursivum by orthogonal design]
Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials
|October 24, 2001
Abstract
Objective:
To select the optimum medium for callus culture of Peucedanum decursivum.
Method:
Orthogonal design was applied in determination of hormonal kinds and concentration, and qualitative analysis of callus was done initially.
Results:
The MS basic medium with 1 mg/L 2,4-D was the best one; coumarin was detected in callus preliminarily.
Conclusion:
It was effective that orthogonal design applied in screening medium; feasible foundation was offered for Peucedanum decursivum cell culture producing active ingredient.
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