Related Experiment Video
Updated: Oct 3, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Solid-Phase Synthesis and Photophysical Properties of DNA Labeled at the Nucleobase with
Shoeb I. Khan1, Amy E. Beilstein, Mark T. Tierney
1Paul M. Gross Chemical Laboratory, Department of Chemistry, Duke University, Durham, North Carolina 27708.
Abstract:
A facile procedure for incorporating a Ru(diimine)(3)(2+) complex at the nucleobase in an oligonucleotide is reported that combines the advantages of Pd(0) cross-coupling and solid-phase DNA chemistries. These ruthenium-modified oligonucleotides form stable duplexes, and the favorable photophysical properties associated with the Ru(diimine)(3)(2+) complex are retained after site-specific covalent attachment.
More Related Videos
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...

