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Summary
This study shows that lysozyme in mouse intestinal fluid originates from Paneth cells. Pilocarpine stimulates the secretion of this lysozyme, highlighting its role in intestinal defense.
Area of Science:
- Gastroenterology
- Immunology
- Cell Biology
Background:
- Paneth cells are specialized epithelial cells located at the base of intestinal crypts.
- Lysozyme is an antimicrobial enzyme found in various bodily secretions.
Purpose of the Study:
- To investigate the origin and regulation of lysozyme in the mouse small intestine.
- To determine if Paneth cells are the source of intestinal lysozyme and if its secretion can be stimulated.
Main Methods:
- Indirect fluorescent antibody technique using an antiserum against mouse intestinal lysozyme.
- Administration of pilocarpine to stimulate secretion.
- Perfusion studies to quantify lysozyme output.
Main Results:
- Fluorescence for lysozyme was localized to Paneth cells in the crypts of Lieberkuhn.
- Pilocarpine administration caused a shift in fluorescence from Paneth cells to the crypt lumen.
- Lysozyme output increased significantly after pilocarpine stimulation, from basal levels of 0.1-0.4 µg/ml to peak rates of 1.8-6.5 µg/ml.
Conclusions:
- Lysozyme in the succus entericus is derived, at least in part, from Paneth cells, likely stored in their granules.
- Pilocarpine stimulates the secretion of Paneth cell lysozyme.
- This research provides a model for studying Paneth cell function within the intestinal defense system.