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An FKBP12 binding assay based upon biotinylated FKBP12

C W Carreras1, H Fu, D V Santi

  • 1Department of Pharmacological Sciences, Kosan Biosciences, Inc., 3832 Bay Center Place, Hayward, California 94545, USA. carreras@kosan.com

Analytical Biochemistry
|October 25, 2001
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...