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Related Experiment Videos

Daily rhythm in human urinary melatonin.

H J Lynch, R J Wurtman, M A Moskowitz

    Science (New York, N.Y.)
    |January 17, 1975
    PubMed
    Summary
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    Urine melatonin levels were significantly higher during nighttime hours (11 p.m. to 7 a.m.) compared to daytime hours in healthy adults. This study quantifies melatonin excretion patterns.

    Area of Science:

    • Biochemistry
    • Chronobiology
    • Endocrinology

    Background:

    • Melatonin is a key hormone regulating circadian rhythms.
    • Understanding melatonin excretion patterns is crucial for chronobiology research.

    Purpose of the Study:

    • To quantify melatonin levels in human urine samples.
    • To investigate the diurnal variation of melatonin excretion.

    Main Methods:

    • Urine samples from six healthy adults were collected over 24-hour periods.
    • Melatonin was concentrated using Amberlite XAD-2 resin and organic solvent elution.
    • Quantification was performed using a bioassay (dermal melanaphore response of larval anurans).

    Main Results:

    • Melatonin levels in urine collected between 11 p.m. and 7 a.m. were several times higher than those collected during daytime hours (7 a.m. - 3 p.m. and 3 p.m. - 11 p.m.).

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  • A clear nocturnal peak in urinary melatonin excretion was observed.

    • Conclusions:

    • Human urinary melatonin excretion exhibits significant diurnal variation, with a pronounced peak during nighttime.
    • These findings support the role of melatonin as a marker for circadian rhythm in humans.