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Solid phase radioimmunossay for serum ferritin.

J W Halliday, K L Gera, L W Powell

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |February 8, 1975
    PubMed
    Summary
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    A new radioimmunoassay accurately measures serum ferritin, a vital protein. This sensitive method aids in diagnosing iron deficiency and other conditions.

    Area of Science:

    • Clinical Chemistry
    • Immunology

    Background:

    • Ferritin is a key protein for iron storage and regulation.
    • Accurate measurement of serum ferritin is crucial for diagnosing various medical conditions, including iron deficiency anemia and inflammatory diseases.

    Purpose of the Study:

    • To describe a novel, highly sensitive, and reproducible solid-phase radioimmunoassay for quantifying human serum ferritin.
    • To establish reference ranges for serum ferritin levels in healthy adult males and females.

    Main Methods:

    • Development of a solid-phase "direct" radioimmunoassay utilizing specific anti-ferritin antibody.
    • The assay demonstrates high sensitivity (detecting < 0.5 ng/ml) and reproducibility.
    • Method allows for storage of coated tubes and automated washing procedures.

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    Main Results:

    • Established normal serum ferritin ranges: 56.9 ng/ml (geometric mean) for males (95% CI: 18-180 ng/ml) and 34.0 ng/ml (geometric mean) for females (95% CI: 10-143 ng/ml).
    • The developed radioimmunoassay is highly sensitive and reproducible.

    Conclusions:

    • The described radioimmunoassay provides a reliable and sensitive method for serum ferritin determination.
    • This assay is suitable for clinical laboratory use and can be adapted for automation.
    • The established reference ranges contribute to the accurate interpretation of ferritin levels in clinical practice.