Related Experiment Video
Updated: Oct 3, 2026

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
Published on: April 23, 2016
Linearization and integration of DNA into cells preferentially occurs at intrinsically curved regions from human
T Kusakabe1, Y Sugimoto, T Maeda
1Laboratory of Silkworm Science, Faculty of Agriculture, Kyusyu University Graduate School, 6-10-1 Hakozaki, Fukuoka 812, Japan. kusakabe@agr.kyushu-u.ac.jp
Abstract:
A bent DNA library was constructed from human genomic DNA, from which a new clone belonging to the human LINE-1 sequence family was isolated and characterized. This clone, with a length of 378 base pairs and termed HBC-1 (human bent clone-1), contained an intrinsically occurring curved DNA structure. By permutation analysis, the center of curvature of this fragment was mapped onto the nucleotide position 886 from the 5' terminus of the complete LINE-1 sequence. Reporter plasmids, which contain HBC-1, were effectively integrated into human chromosome, indicating that the bent DNA structure provides a preferential donor site for the integration of human LINE-1 sequences. The present finding may provide an explanation as to why some inactivated LINE-1 sequences on human chromosomes carry the deletion at their 5' termini.
Related Concept Videos
Homologous Recombination
Homologous Recombination
The DNA Replication Fork
Non-LTR Retrotransposons
Replication in Prokaryotes
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Replication in Prokaryotes
