Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polymorphonuclear leukocytes in antibody-dependent cellular cytotoxicity.

R P Gale, J Zighelboim

    Journal of Immunology (Baltimore, Md. : 1950)
    |March 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Psychological and immunological reactions of family members to patients undergoing bone marrow transplantation.

    Psychosomatic medicine·1996
    Same author

    Methods for studying intestinal sensitivity and compliance: in vitro studies of balloons and a barostat.

    Neurogastroenterology and motility·1996
    Same author

    Bone marrow transplantation for Fanconi anemia.

    Blood·1995
    Same author

    Long-term follow-up of adults with acute lymphoblastic leukemia in first remission treated with chemotherapy or bone marrow transplantation. The Acute Lymphoblastic Leukemia Working Committee.

    Annals of internal medicine·1995
    Same author

    Impact of cytogenetic abnormalities on outcome of bone marrow transplants in acute myelogenous leukemia in first remission.

    Bone marrow transplantation·1995
    Same author

    Chronic myelogenous leukemia as a model of cancer development.

    Seminars in oncology·1995

    Human polymorphonuclear leukocytes (PMN) efficiently lyse antibody-coated cells via antibody-dependent cellular cytotoxicity (ADCC). This rapid PMN-mediated killing mechanism, independent of complement, suggests a significant role in tumor cell immunity.

    Area of Science:

    • Immunology
    • Cell Biology
    • Cancer Research

    Background:

    • Antibody-dependent cellular cytotoxicity (ADCC) is a key immune mechanism.
    • Polymorphonuclear leukocytes (PMN) are crucial innate immune cells.
    • The role of PMN in ADCC against tumor cells requires further elucidation.

    Purpose of the Study:

    • To investigate the capacity of human PMN to mediate ADCC.
    • To characterize the kinetics and efficiency of PMN-mediated ADCC.
    • To explore the potential of PMN in anti-tumor immunity.

    Main Methods:

    • Co-incubation of human PMN with antibody-coated target cells.
    • Quantification of target cell lysis.
    • Assessment of inhibition by aggregated gamma-globulin.

    Related Experiment Videos

    Main Results:

    • Human PMN effectively lyse antibody-coated target cells in an immunologically specific manner (ADCC).
    • PMN-mediated cytolysis is rapid, occurs at low PMN:target cell ratios, and is complement-independent.
    • The process is mediated via Fc receptors and can be inhibited by aggregated gamma-globulin.

    Conclusions:

    • Human PMN are potent mediators of ADCC against antibody-coated cells.
    • PMN-mediated ADCC is a rapid and efficient cytotoxic mechanism.
    • These findings highlight a significant role for PMN in host tumor cell immunity.