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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nuclear Export01:42

Nuclear Export

The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

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Related Experiment Video

Updated: Jun 19, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p.

K Strässer1, E Hurt

  • 1BZH--Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.

Nature
|October 26, 2001
PubMed
Summary
This summary is machine-generated.

The spliceosomal protein Sub2p is crucial for mRNA export in yeast, interacting with Yra1p to facilitate transport. This interaction is key for recruiting Yra1p to mRNA, enabling its nuclear export.

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Last Updated: Jun 19, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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Published on: August 9, 2019

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

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Published on: September 15, 2021

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Yeast nuclear protein Yra1p is essential for messenger RNA (mRNA) export.
  • Yra1p interacts with the mRNA transport factor Mex67p/Mtr2p at the nuclear pore.

Purpose of the Study:

  • Investigate the role of SUB2, a DEAD box helicase involved in splicing, in mRNA export.
  • Elucidate the interaction between Yra1p and Sub2p in the context of mRNA export.

Main Methods:

  • Genetic interaction analysis between YRA1 and SUB2.
  • In vivo and in vitro binding assays to study protein-protein interactions.
  • Analysis of protein binding domains and competition assays.

Main Results:

  • Mutation or overexpression of SUB2 impairs mRNA export.
  • Yra1p and Sub2p directly bind to each other both in vivo and in vitro.
  • Sub2p and Mex67p/Mtr2p compete for binding to the same domains on Yra1p.

Conclusions:

  • The spliceosomal component Sub2p plays a significant role in mRNA export.
  • Sub2p likely recruits Yra1p to mRNA for export.
  • Mex67p/Mtr2p binding to Yra1p may displace Sub2p, facilitating nuclear pore passage.