[Photometric analysis with reaction between metal ion (M) and organic reagent (R)]

Firstly, the syntheses of various organic reagents (R) containing such xanthene derivatives as O-hydroxyhydro-quinonephthalein (QnPh), and 3,4,5,6-tetrahydroxyfluoran (gallein, Gall) were studied. Secondarily, the coexisting effects of surfactant (cationic-, anionic-, amphoteric- and nonionic-surfactants alone or combination) on the coloring or fluorescence reactions between various (R) such as xanthene derivatives, aromatic amines and various metal ions (M) such as bismuth(III), tin(IV), iron(III), molybdenum(VI), uranium(VI), cobalt(II), aluminum(III), etc. were systematically investigated. Thirdly, numerous simple and sensitive photometric analyses (spectrophotometry and fluorophotometry) for various (M) and organic compounds(Org) such as biological samples, and pharmaceutical preparations by formation reactions of (R-M) binary or ((R-M)-Org) ternary complexes were established in the coexistence of surfactant alone or combination.