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Pancreatic enzyme secretion in the conscious rat. Method and application
Pflugers Archiv : European Journal of Physiology
|January 1, 1975
Summary
This study details a new method for separately collecting duodenal juice and gastric content in rats. The technique allows for precise measurement of pancreatic enzyme secretion under various physiological and pharmacological conditions.
Area of Science:
- Physiology
- Pharmacology
- Gastroenterology
Background:
- Accurate measurement of pancreatic secretions is crucial for understanding digestive physiology and disease.
- Existing methods may not allow for separate collection of duodenal and gastric contents in conscious animals.
- Developing a reliable method is essential for studying pancreatic exocrine function.
Purpose of the Study:
- To describe a novel surgical method for the separate collection of duodenal juice and gastric content in conscious rats.
- To characterize the patterns of duodenal secretion under basal and stimulated conditions.
- To evaluate the suitability of this method for physiological and pharmacological investigations.
Main Methods:
- Surgical implantation of metal cannulas into the stomach fundus of conscious rats.
- Insertion of a double lumen tube through the pylorus into the duodenum via the cannula.
- Administration of cholinergic stimuli, secretin, and cholecystokinin-pancreozymin (CCK-PZ) to assess pancreatic enzyme secretion.
- Observation of secretion patterns, flow rates, and enzyme levels (bicarbonate, trypsin, amylase).
Main Results:
- A constant basal flow rate of duodenal volume and enzymes (bicarbonate, trypsin, amylase) was observed.
- Cholinergic stimuli significantly increased enzyme secretion, an effect blocked by atropine.
- Co-administration of secretin and CCK-PZ elicited a strong secretory response.
- Hormonal stimulation showed dose-dependent effects, with optimal responses between 0.5 and 25 U/100 g b.w./hr.
- Narcosis markedly inhibited both resting and stimulated enzyme secretion.
Conclusions:
- The described method enables separate collection of duodenal juice and gastric content in conscious rats.
- This technique is effective for examining physiological and pharmacological influences on pancreatic enzyme secretion.
- The findings provide a foundation for further research into pancreatic exocrine function and related disorders.