Gas chromatographic-mass spectrometric method for quantitation of phenylalanine and tyrosine in serum
J B Laurens1, X Y Mbianda, J B Ubbink
1Department of Chemical Pathology, Faculty of Medicine, University of Pretoria, South Africa. jlaurens@medic.up.ac.za
Abstract:
A validated gas chromatographic-mass spectrometric method for quantitation of phenylalanine and tyrosine in serum is described. Quantitation of phenylalanine and tyrosine with a non-labelled non-endogenous internal standard, L-2-chlorophenylalanine, compared favourably with isotope dilution mass spectrometric quantitation. The 95% reference ranges for phenylalanine. tyrosine and the phenylalanine-tyrosine molar ratio in neonate cord blood serum were determined by isotope dilution mass spectrometry and were found to be 77.1-144.7, 33.3-109.3 micromol/l and 1.1-3.0, respectively.
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