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Erythrocyte binding antigen (EBA-175) of Plasmodium falciparum: improved genotype determination by nested polymerase
F S Touré1, E Mavoungou, J M Ndong
1Centre International de Recherches Médicales de Franceville, Franceville, Gabon. toure@cirmf.sc.ga
Tropical Medicine & International Health : TM & IH
|October 27, 2001
Abstract:
We have designed primers to the conserved region of the erythrocyte binding antigen (EBA)-175 gene which amplify specifically the two alleles by polymerase chain reaction (PCR) and by nested PCR. This approach provides a specific, sensitive and rapid method for genotype determination in a large number of Plasmodium falciparum field isolates.