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Related Experiment Video

Updated: Jun 9, 2026

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
08:29

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel

Published on: May 14, 2018

Continuous-flow fluoro-immunosensor for paclitaxel measurement.

S H Sheikh1, A Mulchandani

  • 1Department of Chemical and Environmental Engineering, University of California, Riverside CA 92521, USA.

Biosensors & Bioelectronics
|October 27, 2001
PubMed
Summary
This summary is machine-generated.

A new fluorescence immunosensor enables rapid and accurate paclitaxel detection. This continuous-flow system uses antibody immobilization and competitive binding for sensitive quantification in human plasma.

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Immunosensing Technology

Background:

  • Paclitaxel is a crucial chemotherapy drug with a narrow therapeutic index, necessitating precise monitoring.
  • Existing methods for paclitaxel determination can be time-consuming and complex.
  • Development of rapid, sensitive, and accurate analytical tools is essential for effective patient treatment.

Purpose of the Study:

  • To develop a novel fluorescence-based continuous-flow immunosensor for sensitive and rapid paclitaxel determination.
  • To optimize the immunosensor for high precision, accuracy, and reproducibility.
  • To evaluate the immunosensor's performance and applicability in complex biological matrices like human plasma.

Main Methods:

  • Immobilization of anti-paclitaxel antibody onto a glass capillary column via protein A crosslinking.
  • Saturation of antibody binding sites with rhodamine-labeled paclitaxel.
  • Competitive displacement assay using a flow-through spectrofluorometer to detect unbound labeled paclitaxel.
  • Regeneration of the antibody binding sites for continuous use.

Main Results:

  • The developed immunosensor demonstrated high sensitivity, with detection limits as low as 1 ng/ml.
  • Assay linearity was observed up to 100 ng/ml with excellent reproducibility (RSD=4.8%).
  • Rapid assay times, ranging from 2 to 8 minutes, were achieved.
  • Successful application in determining paclitaxel concentrations in human plasma samples.

Conclusions:

  • The fluorescence-based continuous-flow immunosensor provides a sensitive, precise, accurate, and fast method for paclitaxel quantification.
  • The system's ability to regenerate antibody binding sites enhances its economic viability for clinical applications.
  • This immunosensor represents a promising tool for therapeutic drug monitoring of paclitaxel.