A versatile biosensor device for continuous biomedical monitoring.

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Sensor Technology

Background:

• In vivo biosensor performance is limited by fouling and blood clotting, necessitating interfaces like ultrafiltration or microdialysis.
• Quantitative analyte recovery requires submicrolitre sampling, driving the need for small, versatile biosensor devices.

Purpose of the Study:

• To present a miniaturized biosensor device with a pulse-free pump and a nanoliter internal volume.
• To detail the production and construction of the biosensor's flow-through cell.
• To evaluate permselective membranes and develop an in situ electropolymerisation protocol for biological component immobilization.

Main Methods:

• Development and production of a miniaturized biosensor with a 10-20 nl internal volume and a pulse-free pump.
• Construction and optimization of a nanoliter flow-through cell.
• Testing of permselective membranes for bio-selector function.
• In situ electropolymerisation for immobilizing biological components.
• In vivo studies for monitoring glucose and lactate.

Main Results:

• A detailed protocol for producing a nanoliter flow-through cell was established.
• Permselective membranes were evaluated for their performance characteristics.
• Biosensors utilizing in situ electropolymerisation demonstrated successful monitoring of glucose and lactate.
• In vivo data from clinical applications validated the biosensor's utility.

Conclusions:

• The developed miniaturized biosensor device is suitable for direct connection to various interfaces.
• The nanoliter flow-through cell design and electropolymerisation protocol enable effective in vivo monitoring.
• This technology holds promise for advancing continuous biomedical monitoring applications.