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Surface plasmon resonance sensor using molecularly imprinted polymer for detection of sialic acid
1Laboratory of Synthetic Biochemistry, Faculty of Information Sciences, Hiroshima City University, Asaminami-ku, Hiroshima 731-3194, Japan.
Biosensors & Bioelectronics
|October 27, 2001
Abstract:
A surface plasmon resonance (SPR) sensor using a molecularly imprinted polymer-coated sensor chip for the detection of sialic acid was developed. The thinly coated polymer was prepared by co-polymerizing N,N,N-trimethylaminoethyl methacrylate, 2-hydroxyethyl methacrylate and ethyleneglycol dimethacrylate in the presence of p-vinylbenzeneboronic acid ester with sialic acid. The sensor showed a selective response to ganglioside of which sialic acid is located at the non-reducing end and gave a linear relationship from 0.1 to 1.0 mg of ganglioside.