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Updated: Oct 3, 2026

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Published on: December 30, 2016
The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge
M Milani1, L Andolfi, S Cannistraro
1Department of Physics-INFM, c/o Advanced Biotechnology Center-IST, University of Genova, Largo Rosanna Benzi 10, 16132 Genova, Italy.
Abstract:
Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit.
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