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Updated: Aug 3, 2026
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Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
Published on: September 12, 2015
[Microinfusion experiment for mice and its application to pharmacological studies]
1Gene Discovery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. m.ikemoto@aist.go.jp
Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica
|October 30, 2001
Summary
Microinfusion experiments are crucial for understanding protein functions in vivo. Infusing Secreted Protein Acidic and Rich in Cysteine (SPARC) into mouse amygdala enhanced locomotor activity, demonstrating this technique's utility.
Area of Science:
- Molecular Biology
- Neuroscience
- Biochemistry
Context:
- Understanding the functions of uncharacterized proteins is essential for deciphering complex life processes.
- Protein function and network analysis are key to advancing biological research.
- In vivo protein function analysis is increasingly reliant on advanced experimental methods.
Purpose:
- To provide a practical manual for performing microinfusion experiments in mice.
- To demonstrate the utility of microinfusion for in vivo protein function analysis.
- To investigate the effect of Secreted Protein Acidic and Rich in Cysteine (SPARC) on mouse behavior.
Summary:
- This study details a practical protocol for microinfusion experiments in mice.
- Researchers infused Secreted Protein Acidic and Rich in Cysteine (SPARC) into the basolateral amygdala of mice.
- The experiment revealed significantly enhanced locomotor activity following SPARC infusion.
Impact:
- Microinfusion offers a valuable method for exploring uncharacterized protein functions in vivo.
- The findings highlight the potential of microinfusion for behavioral neuroscience research.
- This work serves as a guide for researchers utilizing microinfusion techniques.
