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Oxidative stress markers in pre-uremic patients
M Annuk1, B Fellström, O Akerblom
1Department of Medical Sciences, University Hospital, Uppsala, Sweden. Margus.Annuk@medsci.uu.se
Clinical Nephrology
|October 30, 2001
Summary
Patients with chronic renal failure (CRF) exhibit increased oxidative stress (OS) markers. Key indicators like oxidized glutathione (GSSG) and lipoprotein oxidation resistance correlate with renal failure severity.
Area of Science:
- Biochemistry
- Nephrology
- Oxidative Stress Research
Background:
- Chronic renal failure (CRF) is associated with complex physiological changes.
- Oxidative stress (OS) is implicated in the pathogenesis of various chronic diseases, including kidney disease.
Purpose of the Study:
- To investigate a panel of oxidative stress markers in patients with chronic renal failure (CRF).
- To determine the relationship between specific OS markers and the degree of renal failure in CRF patients.
Main Methods:
- Blood samples were collected from 38 CRF patients and 61 healthy controls.
- Plasma and serum levels of various OS markers including glutathione redox status and lipoprotein oxidation resistance were measured using commercial kits.
Main Results:
- CRF patients showed significantly elevated levels of diene conjugates (DC), baseline diene conjugation of lipoprotein fraction (BDC-LPF), lipid hydroperoxides (LOOH), oxidized glutathione (GSSG), and the GSSG/GSH ratio.
- A significantly shortened lag phase of lipoprotein fraction (LPF) oxidation was observed in CRF patients.
- Serum creatinine and urea levels correlated significantly with GSSG and GSSG/GSH, and inverse correlations were found between glutathione redox ratio and lag phase of LPF, and GSSG level and BDC-LPF.
Conclusions:
- Patients with CRF are in a state of significant oxidative stress compared to healthy individuals.
- GSSG level, GSSG/GSH status, lag phase of LPF, and BDC-LPF are identified as the most informative markers for evaluating the degree of OS in CRF.