Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Physicochemical properties of chromatin digested with trypsin.

N Sugano, S Okada

    Biochimica Et Biophysica Acta
    |February 24, 1975
    PubMed
    Summary

    Tryptic digestion alters chromatin structure, increasing viscosity and DNAase II susceptibility. Hydrophobic amino acids decrease, suggesting their role in maintaining chromatin's tertiary structure.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Usefulness of the one-step technique in functional end-to-end anastomosis for colonic surgery: results of a prospective multicentre cohort study from the Japanese KYCC group.

    Techniques in coloproctology·2024
    Same author

    Application and outcomes of a standardized lymphadenectomy in laparoscopic right hemicolectomy requiring ligation of the middle colic artery.

    Techniques in coloproctology·2021
    Same author

    Femoral morphology asymmetry in hip dysplasia makes radiological leg length measurement inaccurate.

    The bone & joint journal·2019
    Same author

    Assessing the progression of chronic periodontitis using subgingival pathogen levels: a 24-month prospective multicenter cohort study.

    BMC oral health·2017
    Same author

    Salivary pathogen and serum antibody to assess the progression of chronic periodontitis: a 24-mo prospective multicenter cohort study.

    Journal of periodontal research·2016
    Same author

    Chemically driven tetragonal-to-monoclinic polymorphic transformation in retrieved ZTA femoral heads from dual mobility hip implants.

    Journal of the mechanical behavior of biomedical materials·2015

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Structural Biology

    Background:

    • Chromatin, the complex of DNA and proteins that forms chromosomes within the nucleus of eukaryotic cells, plays a crucial role in genome organization and regulation.
    • Understanding the structural dynamics of chromatin is essential for comprehending gene expression and cellular processes.

    Purpose of the Study:

    • To investigate the physicochemical changes in calf thymus chromatin upon tryptic digestion.
    • To elucidate the role of specific amino acid compositions in maintaining chromatin's tertiary structure.

    Main Methods:

    • Preparation and tryptic digestion of calf thymus chromatin.
    • Measurement of chromatin viscosity and susceptibility to DNAase II.
    • Analysis of thermal denaturation profiles (melting temperature).
    • Amino acid composition analysis (basic and hydrophobic amino acids).

    Main Results:

    • Tryptic digestion increased chromatin viscosity and DNAase II susceptibility.
    • Thermal denaturation shifted from biphasic (native) to monophasic (digested), with a significant decrease in melting temperature.
    • The molar percentage of basic amino acids increased, while hydrophobic amino acids decreased with digestion time.
    • The ratio of hydrophobic to basic amino acids decreased significantly after 180 minutes of tryptic digestion.

    Conclusions:

    • Tryptic digestion alters chromatin's physicochemical properties, including its thermal stability and susceptibility to enzymatic degradation.
    • The observed changes suggest that hydrophobic portions of chromatin proteins are critical for maintaining the tertiary structure of chromatin.

    Related Experiment Videos