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HBP2: a new mammalian protein that complements the fission yeast MBF transcription complex
A Sánchez-Díaz1, M A Blanco, N Jones
1Instituto de Microbiologia Bioquimica, Departamento de Microbiologia y Genética, CSIC/Universidad de Salamanca, Spain.
Abstract:
The mammalian HBP2 gene has been isolated by functional complementation of cells unable to undergo DNA replication in fission yeast. HBP2 is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As in other members of the family, the HMG box in HBP2 is a DNA-binding domain that is essential for its function in Schizosaccharomyces pombe. Expression of HBP2 in fission yeast activates CdclO-dependent transcription at G1/S and allows growth of cdc10-129, resldelta and rep2delta mutant cells at the restrictive temperature. The mammalian HBP2 activates transcription at G1/S in the fission yeast Sch. pombe.