Consideration on peak shape in a batch-type chemiluminescence detection cell for capillary electrophoresis

Peak areas, peak heights, and apparent theoretical plate numbers were examined as a function of sample injection times by use of the batch-type CL detection cell. Comparing the experimental data with those obtained by absorption detector, some considerations were carried out about the peak shape. The peak shape in CL detection was influenced by not only concentration distribution of sample in a sample zone but also sample diffusion and CL reaction at the capillary outlet. The sample injection time of ca. 35 s was recommended for the present CE with CL detector. The injection time much influenced peak shape as well as sensitivity in the CL detection cell.