Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Construction of a reference linkage map for melon.

M Oliver1, J Garcia-Mas, M Cardús

  • 1Institut de Recerca i Tecnologia Agroalimentàries, Departament de Genètica Vegetal, Cabrils, Barcelona, Spain.

Genome
|October 30, 2001
PubMed
Summary

A comprehensive genetic map of melon (Cucumis melo L.) was developed using 411 markers. This map aids in understanding melon genetics and facilitates comparisons with other cucurbit genetic maps.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measuring the principal Hugoniot of inertial-confinement-fusion-relevant TMPTA plastic foams.

Physical review. E·2023
Same author

Developing a platform for Fresnel diffractive radiography with 1 μm spatial resolution at the National Ignition Facility.

The Review of scientific instruments·2023
Same author

Diffraction enhanced imaging utilizing a laser produced x-ray source.

The Review of scientific instruments·2022
Same author

Stabilized Radiation Pressure Acceleration and Neutron Generation in Ultrathin Deuterated Foils.

Physical review letters·2022
Same author

Toward an integrated platform for characterizing laser-driven, isochorically heated plasmas with 1  µm spatial resolution.

Applied optics·2022
Same author

Peripheral blood lymphocyte phenotypes in Alzheimer and Parkinson's diseases.

Neurologia·2022

Area of Science:

  • Plant Genetics
  • Molecular Biology
  • Agricultural Science

Background:

  • Developing detailed genetic maps is crucial for crop improvement in melon (Cucumis melo L.).
  • Previous genetic mapping efforts in melon have provided foundational data but require further refinement and integration.

Purpose of the Study:

  • To construct a high-density genetic map for melon (Cucumis melo L.) utilizing a diverse set of molecular markers.
  • To identify potential anchor points for comparative mapping within the Cucurbitaceae family.

Main Methods:

  • A genetic map was constructed using the F2 progeny from a cross between a Korean accession and the Spanish melon type 'Pinyonet Piel de Sapo'.
  • A total of 411 markers were employed, including Restriction Fragment Length Polymorphisms (RFLPs), Amplified Fragment Length Polymorphisms (AFLPs), Random Amplified Polymorphic DNAs (RAPDs), Simple Sequence Repeats (SSRs), inter-SSRs, and isozymes, along with one morphological trait (carpel number).

Related Experiment Videos

  • Restriction Fragment Length Polymorphisms (RFLPs) were generated using probes from melon genomic and cDNA libraries, including Arabidopsis ESTs and known Cucumis genes.

    • Main Results:

    • The map comprises 391 loci distributed across 12 major linkage groups, covering a genetic distance of 1197 cM with an average marker interval of 3 cM.
    • A majority of the markers (66%) were codominant (RFLPs, SSRs, isozymes), enhancing their utility and transferability across different melon crosses.
    • Twenty-one loci (six RAPDs and 15 AFLPs) did not show linkage within the established map.

    Conclusions:

    • The developed melon genetic map provides a valuable resource for future genetic studies and marker-assisted selection in melon breeding.
    • The identified codominant markers and potential anchor points facilitate the integration of this map with other existing melon and cucumber genetic maps.
    • This research contributes to a better understanding of melon genome organization and enables comparative genomics within the cucurbit family.