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Updated: Oct 3, 2026
Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety
Published on: July 24, 2011
Gene transfer using human papillomavirus pseudovirions varies according to virus genotype and requires cell surface
A L Combita1, A Touzé, L Bousarghin
1Laboratoire de Virologie Moléculaire, INSERM EMIU 00-10, Faculté des Sciences Pharmaceutiques P. Maupas, 31 avenue Monge, 37200 Tours, France.
Abstract:
Artificial viruses consisting of DNA plasmid packaged in vitro into virus-like particles (VLPs) are new vehicles for gene transfer. We therefore investigated the ability of nine human papillomavirus (HPV) VLPs to interact with heterologous DNA and transfer genes. HPV 16, 18, 31, 33, 39, 45, 58, 59, and 68 VLPs were able to bind heterologous DNA and to transfer genes into Cos-7 cells. Inhibition of gene transfer by preincubation of the pseudovirions with heparin confirmed that heparan sulfate on the cell surface plays a role as cell receptor for HPVs. As HPV neutralizing antibodies are mainly type-specific, gene transfer with different HPV pseudovirions offers the possibility of their sequential use in vivo for a greater efficacy.
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